SEQUENCE LISTING

<110>	BHARAT SERUMS AND VACCINES LTD.
<120>	ANTI-RHD MONOCLONCAL ANTIBODIES
<130>	PCT-ANTID-01
<150>	2730/MUM/2008
<151>	31ST DECEMBER 2008
<160>	48

SEQ ID NO: 1 (Nucleotide sequence of the coding region of RhD1 HC) 

Signal peptide:	1-57
Variable region:	58-448
CDR1:			133-156
CDR2:			208-231
CDR3:			346-414
Constant region:	449-1437 (gamma1)

	 M   D   W   T   W   R   F   L   F   V   V   A   A   A   T   G   V   Q   S   Q
       1	ATG GAC TGG ACC TGG AGG TTC CTC TTT GTG GTG GCA GCA GCT ACA GGT GTC CAG TCC CAG

	 V   Q   L   V   Q   S   G   A   E   V   K   K   P   G   S   S   V   K   V   S
      61	GTG CAG CTG GTG CAG TCT GGG GCT GAG GTG AAG AAG CCT GGG TCC TCG GTG AAG GTC TCC

	 C   K   A   S   G   G   I   F   R   T   Y   A   I   S   W   V   R   Q   A   P
     121	TGC AAG GCT TCT GGA GGC ATC TTC AGA ACC TAT GCT ATC AGC TGG GTG CGA CAG GCC CCT

	 G   Q   G   L   E   W   M   G   G   I   I   P   M   F   G   T   V   N   Y   A
     181	GGA CAA GGG CTT GAG TGG ATG GGA GGG ATC ATC CCT ATG TTT GGT ACA GTA AAC TAC GCA

	 Q   K   F   Q   G   R   V   T   I   S   A   D   K   S   T   S   T   A   Y   M
     241	CAG AAG TTC CAG GGC AGA GTC ACG ATT AGC GCG GAC AAA TCC ACG AGC ACA GCC TAT ATG

	 E   L   S   R   L   R   S   E   D   T   A   V   Y   Y   C   A   R   P   P   S
     301	GAA CTG AGC AGA CTG AGA TCT GAG GAC ACG GCC GTG TAT TAC TGT GCG AGG CCG CCT TCC

	 G   G   C   G   G   D   C   S   R   R   G   Y   Y   Y   A   M   D   V   W   G
     361	GGG GGT TGT GGT GGT GAC TGC TCA CGG AGG GGC TAC TAC TAC GCC ATG GAC GTC TGG GGC

	 Q   G   T   T   I   T   V   S   S   A   S   T   K   G   P   S   V   F   P   L
     421	CAA GGG ACC ACG ATC ACC GTC TCC TCA GCC TCC ACC AAG GGC CCA TCG GTC TTC CCC CTG

	 A   P   S   S   K   S   T   S   G   G   T   A   A   L   G   C   L   V   K   D
     481	GCA CCC TCC TCC AAG AGC ACC TCT GGG GGC ACA GCG GCC CTG GGC TGC CTG GTC AAG GAC

	 Y   F   P   E   P   V   T   V   S   W   N   S   G   A   L   T   S   G   V   H
     541	TAC TTC CCC GAA CCG GTG ACG GTG TCG TGG AAC TCA GGC GCC CTG ACC AGC GGC GTG CAC

	 T   F   P   A   V   L   Q   S   S   G   L   Y   S   L   S   S   V   V   T   V
     601	ACC TTC CCG GCT GTC CTA CAG TCC TCA GGA CTC TAC TCC CTC AGC AGC GTG GTG ACC GTG

	 P   S   S   S   L   G   T   Q   T   Y   I   C   N   V   N   H   K   P   S   N
     661	CCC TCC AGC AGC TTG GGC ACC CAG ACC TAC ATC TGC AAC GTG AAT CAC AAG CCC AGC AAC

	 T   K   V   D   K   K   V   E   P   K   S   C   D   K   T   H   T   C   P   P
     721	ACC AAG GTG GAC AAG AAA GTT GAG CCC AAA TCT TGT GAC AAA ACT CAC ACA TGC CCA CCG

	 C   P   A   P   E   L   L   G   G   P   S   V   F   L   F   P   P   K   P   K
     781	TGC CCA GCA CCT GAA CTC CTG GGG GGA CCG TCA GTC TTC CTC TTC CCC CCA AAA CCC AAG

	 D   T   L   M   I   S   R   T   P   E   V   T   C   V   V   V   D   V   S   H
     841	GAC ACC CTC ATG ATC TCC CGG ACC CCT GAG GTC ACA TGC GTG GTG GTG GAC GTG AGC CAC

	 E   D   P   E   V   K   F   N   W   Y   V   D   G   V   E   V   H   N   A   K
     901	GAA GAC CCT GAG GTC AAG TTC AAC TGG TAC GTG GAC GGC GTG GAG GTG CAT AAT GCC AAG

	 T   K   P   R   E   E   Q   Y   N   S   T   Y   R   V   V   S   V   L   T   V
     961	ACA AAG CCG CGG GAG GAG CAG TAC AAC AGC ACG TAC CGT GTG GTC AGC GTC CTC ACC GTC


	 L   H   Q   D   W   L   N   G   K   E   Y   K   C   K   V   S   N   K   A   L
    1021	CTG CAC CAG GAC TGG CTG AAT GGC AAG GAG TAC AAG TGC AAG GTC TCC AAC AAA GCC CTC

	 P   A   P   I   E   K   T   I   S   K   A   K   G   Q   P   R   E   P   Q   V
    1081	CCA GCC CCC ATC GAG AAA ACC ATC TCC AAA GCC AAA GGG CAG CCC CGA GAA CCA CAG GTG


	 Y   T   L   P   P   S   R   D   E   L   T   K   N   Q   V   S   L   T   C   L
    1141	TAC ACC CTG CCC CCA TCC CGG GAT GAG CTG ACC AAG AAC CAG GTC AGC CTG ACC TGC CTG

	 V   K   G   F   Y   P   S   D   I   A   V   E   W   E   S   N   G   Q   P   E
    1201	GTC AAA GGC TTC TAT CCC AGC GAC ATC GCC GTG GAG TGG GAG AGC AAT GGG CAG CCG GAG

	 N   N   Y   K   T   T   P   P   V   L   D   S   D   G   S   F   F   L   Y   S
    1261	AAC AAC TAC AAG ACC ACG CCT CCC GTG CTG GAC TCC GAC GGC TCC TTC TTC CTC TAC AGC

	 K   L   T   V   D   K   S   R   W   Q   Q   G   N   V   F   S   C   S   V   M
    1321	AAG CTC ACC GTG GAC AAG AGC AGG TGG CAG CAG GGG AAC GTC TTC TCA TGC TCC GTG ATG

	 H   E   A   L   H   N   H   Y   T   Q   K   S   L   S   L   S   P   G   K   *
    1381	CAT GAG GCT CTG CAC AAC CAC TAC ACG CAG AAG AGC CTC TCC CTG TCT CCG GGT AAA TGA


SEQ ID NO: 2 (RhD1 HC amino acid sequence)

       1	MDWTWRFLFV VAAATGVQSQ VQLVQSGAEV KKPGSSVKVS CKASGGIFRT YAISWVRQAP
      61	GQGLEWMGGI IPMFGTVNYA QKFQGRVTIS ADKSTSTAYM ELSRLRSEDT AVYYCARPPS
     121	GGCGGDCSRR GYYYAMDVWG QGTTITVSSA STKGPSVFPL APSSKSTSGG TAALGCLVKD
     181	YFPEPVTVSW NSGALTSGVH TFPAVLQSSG LYSLSSVVTV PSSSLGTQTY ICNVNHKPSN
     241	TKVDKKVEPK SCDKTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH
     301	EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL
     361	PAPIEKTISK AKGQPREPQV YTLPPSRDEL TKNQVSLTCL VKGFYPSDIA VEWESNGQPE
     421	NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGK


SEQ ID NO: 3 (Nucleotide sequence of the coding region of RhD1 LC)

Signal peptide:	1-57
Variable region:	58-388
CDR1:			133-159
CDR2:			211-219
CDR3:			328-357
Constant region:	389-705 (lambda)

	 M   A   W   A   L   L   F   L   T   L   L   T   Q   G   T   G   S   W   A   Q
       1	ATG GCC TGG GCT CTG CTA TTC CTC ACC CTC CTC ACT CAG GGC ACA GGG TCC TGG GCC CAG

	 S   A   L   T   Q   P   A   S   V   S   G   S   P   G   Q   S   I   T   I   S
      61	TCT GCC CTG ACT CAA CCT GCC TCC GTG TCT GGG TCT CCT GGA CAG TCG ATC ACC ATC TCC

	 C   S   G   S   S   S   D   V   G   G   Y   K   Y   V   S   W   Y   Q   Q   H
     121	TGC AGT GGA AGC AGC AGT GAC GTT GGT GGT TAT AAG TAT GTC TCC TGG TAC CAA CAA CAC

	 P   G   K   A   P   Q   L   M   I   Y   D   V   N   N   R   P   S   G   V   S
     181	CCA GGC AAA GCC CCC CAA CTC ATG ATT TAT GAT GTC AAT AAT CGG CCC TCA GGG GTT TCT

	 N   R   F   S   G   S   K   S   G   N   T   A   S   L   T   I   S   G   L   Q
     241	AAT CGC TTC TCT GGC TCC AAG TCT GGC AAC ACG GCC TCC CTG ACC ATC TCT GGG CTC CAG

	 A   E   D   E   A   D   Y   Y   C   S   S   Y   T   S   S   S   T   R   V   F
     301	GCT GAG GAC GAG GCT GAT TAT TAC TGC AGC TCA TAT ACA AGC AGC AGC ACT CGA GTG TTC

	 G   G   G   T   K   L   T   V   L   G   Q   P   K   A   A   P   S   V   T   L
     361	GGC GGA GGG ACG AAG CTG ACC GTC CTA GGT CAG CCC AAG GCT GCC CCC TCG GTC ACT CTG

	 F   P   P   S   S   E   E   L   Q   A   N   K   A   T   L   V   C   L   I   S
     421	TTC CCA CCC TCC TCT GAG GAG CTT CAA GCC AAC AAG GCC ACA CTG GTG TGT CTC ATA AGT

	 D   F   Y   P   G   A   V   T   V   A   W   K   A   D   S   S   P   V   K   A
     481	GAC TTC TAC CCG GGA GCC GTG ACA GTG GCC TGG AAG GCA GAT AGC AGC CCC GTC AAG GCG
	 G   V   E   T   T   T   P   S   K   Q   S   N   N   K   Y   A   A   S   S   Y
     541	GGA GTG GAG ACC ACC ACA CCC TCC AAA CAA AGC AAC AAC AAG TAC GCG GCC AGC AGC TAC

	 L   S   L   T   P   E   Q   W   K   S   H   K   S   Y   S   C   Q   V   T   H
     601	CTG AGC CTG ACG CCT GAG CAG TGG AAG TCC CAC AAA AGC TAC AGC TGC CAG GTC ACG CAT

	 E   G   S   T   V   E   K   T   V   A   P   T   E   C   S   *
     661	GAA GGG AGC ACC GTG GAG AAG ACA GTG GCC CCT ACA GAA TGT TCA TAG

SEQ ID NO: 4 (RhD1 LC: amino acid sequence)

       1	MAWALLFLTL LTQGTGSWAQ SALTQPASVS GSPGQSITIS CSGSSSDVGG YKYVSWYQQH
      61	PGKAPQLMIY DVNNRPSGVS NRFSGSKSGN TASLTISGLQ AEDEADYYCS SYTSSSTRVF
     121	GGGTKLTVLG QPKAAPSVTL FPPSSEELQA NKATLVCLIS DFYPGAVTVA WKADSSPVKA
     181	GVETTTPSKQ SNNKYAASSY LSLTPEQWKS HKSYSCQVTH EGSTVEKTVA PTECS


SEQ ID NO: 5 (Nucleotide sequence of the coding region of RhD2 HC)

Signal peptide:	1-57
Variable region:	58-448
CDR1:			133-156
CDR2:			208-231
CDR3:			346-414
Constant region:	449-1437 (gamma1)

	 M   D   W   T   W   R   F   L   F   V   V   A   A   A   T   G   V   Q   S   Q
       1	ATG GAC TGG ACC TGG AGG TTC CTC TTT GTG GTG GCA GCA GCT ACA GGT GTC CAG TCC CAG

	 V   Q   L   V   Q   S   G   A   E   V   K   K   P   G   S   S   V   K   V   S
      61	GTG CAG CTG GTG CAG TCT GGG GCT GAG GTG AAG AAG CCT GGG TCC TCG GTG AAG GTC TCC

	 C   K   P   S   G   G   I   F   S   T   Y   A   I   S   W   V   R   Q   A   P
     121	TGC AAA CCT TCT GGA GGC ATC TTC AGC ACC TAT GCT ATC AGC TGG GTG CGA CAG GCC CCG

	 G   Q   G   L   E   W   M   G   G   I   I   P   M   F   G   T   V   N   Y   A
     181	GGA CAA GGG CTT GAG TGG ATG GGA GGG ATC ATC CCT ATG TTT GGG ACA GTA AAC TAC GCA

	 Q   K   F   Q   G   R   V   T   I   S   A   G   K   S   T   S   T   A   D   M
     241	CAG AAG TTC CAG GGC AGA GTC ACC ATT AGC GCG GGC AAA TCC ACG AGC ACA GCC GAT ATG

	 E   L   S   R   L   R   S   E   D   T   A   V   Y   Y   C   A   R   P   P   S
     301	GAA CTG AGC AGA CTG AGA TCT GAG GAC ACG GCC GTG TAT TAC TGT GCG AGG CCG CCT TCC

	 G   G   C   G   G   D   C   S   R   R   G   Y   Y   Y   G   M   D   V   W   G
     361	GGG GGT TGT GGT GGT GAC TGC TCA CGG AGG GGC TAT TAT TAT GGT ATG GAC GTC TGG GGC

	 Q   G   T   T   V   I   V   S   S   A   S   T   K   G   P   S   V   F   P   L
     421	CAA GGG ACC ACG GTC ATC GTC TCC TCA GCC TCC ACC AAG GGC CCA TCG GTC TTC CCC CTG

	 A   P   S   S   K   S   T   S   G   G   T   A   A   L   G   C   L   V   K   D
     481	GCA CCC TCC TCC AAG AGC ACC TCT GGG GGC ACA GCG GCC CTG GGC TGC CTG GTC AAG GAC

	 Y   F   P   E   P   V   T   V   S   W   N   S   G   A   L   T   S   G   V   H
     541	TAC TTC CCC GAA CCG GTG ACG GTG TCG TGG AAC TCA GGC GCC CTG ACC AGC GGC GTG CAC

	 T   F   P   A   V   L   Q   S   S   G   L   Y   S   L   S   S   V   V   T   V
     601	ACC TTC CCG GCT GTC CTA CAG TCC TCA GGA CTC TAC TCC CTC AGC AGC GTG GTG ACC GTG

	 P   S   S   S   L   G   T   Q   T   Y   I   C   N   V   N   H   K   P   S   N
     661	CCC TCC AGC AGC TTG GGC ACC CAG ACC TAC ATC TGC AAC GTG AAT CAC AAG CCC AGC AAC

	 T   K   V   D   K   K   V   E   P   K   S   C   D   K   T   H   T   C   P   P
     721	ACC AAG GTG GAC AAG AAA GTT GAG CCC AAA TCT TGT GAC AAA ACT CAC ACA TGC CCA CCG


	 C   P   A   P   E   L   L   G   G   P   S   V   F   L   F   P   P   K   P   K
     781	TGC CCA GCA CCT GAA CTC CTG GGG GGA CCG TCA GTC TTC CTC TTC CCC CCA AAA CCC AAG

	 D   T   L   M   I   S   R   T   P   E   V   T   C   V   V   V   D   V   S   H
     841	GAC ACC CTC ATG ATC TCC CGG ACC CCT GAG GTC ACA TGC GTG GTG GTG GAC GTG AGC CAC

	 E   D   P   E   V   K   F   N   W   Y   V   D   G   V   E   V   H   N   A   K
     901	GAA GAC CCT GAG GTC AAG TTC AAC TGG TAC GTG GAC GGC GTG GAG GTG CAT AAT GCC AAG

	 T   K   P   R   E   E   Q   Y   N   S   T   Y   R   V   V   S   V   L   T   V
     961	ACA AAG CCG CGG GAG GAG CAG TAC AAC AGC ACG TAC CGT GTG GTC AGC GTC CTC ACC GTC

	 L   H   Q   D   W   L   N   G   K   E   Y   K   C   K   V   S   N   K   A   L
    1021	CTG CAC CAG GAC TGG CTG AAT GGC AAG GAG TAC AAG TGC AAG GTC TCC AAC AAA GCC CTC

	 P   A   P   I   E   K   T   I   S   K   A   K   G   Q   P   R   E   P   Q   V
    1081	CCA GCC CCC ATC GAG AAA ACC ATC TCC AAA GCC AAA GGG CAG CCC CGA GAA CCA CAG GTG

	 Y   T   L   P   P   S   R   D   E   L   T   K   N   Q   V   S   L   T   C   L
    1141	TAC ACC CTG CCC CCA TCC CGG GAT GAG CTG ACC AAG AAC CAG GTC AGC CTG ACC TGC CTG


	 V   K   G   F   Y   P   S   D   I   A   V   E   W   E   S   N   G   Q   P   E
    1201	GTC AAA GGC TTC TAT CCC AGC GAC ATC GCC GTG GAG TGG GAG AGC AAT GGG CAG CCG GAG

	 N   N   Y   K   T   T   P   P   V   L   D   S   D   G   S   F   F   L   Y   S
    1261	AAC AAC TAC AAG ACC ACG CCT CCC GTG CTG GAC TCC GAC GGC TCC TTC TTC CTC TAC AGC

	 K   L   T   V   D   K   S   R   W   Q   Q   G   N   V   F   S   C   S   V   M
    1321	AAG CTC ACC GTG GAC AAG AGC AGG TGG CAG CAG GGG AAC GTC TTC TCA TGC TCC GTG ATG

	 H   E   A   L   H   N   H   Y   T   Q   K   S   L   S   L   S   P   G   K   *
    1381	CAT GAG GCT CTG CAC AAC CAC TAC ACG CAG AAG AGC CTC TCC CTG TCT CCG GGT AAA TGA



SEQ ID NO: 6 (RhD2 HC: amino acid sequence)

       1	MDWTWRFLFV VAAATGVQSQ VQLVQSGAEV KKPGSSVKVS CKPSGGIFST YAISWVRQAP
      61	GQGLEWMGGI IPMFGTVNYA QKFQGRVTIS AGKSTSTADM ELSRLRSEDT AVYYCARPPS
     121	GGCGGDCSRR GYYYGMDVWG QGTTVIVSSA STKGPSVFPL APSSKSTSGG TAALGCLVKD
     181	YFPEPVTVSW NSGALTSGVH TFPAVLQSSG LYSLSSVVTV PSSSLGTQTY ICNVNHKPSN
     241	TKVDKKVEPK SCDKTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH
     301	EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL
     361	PAPIEKTISK AKGQPREPQV YTLPPSRDEL TKNQVSLTCL VKGFYPSDIA VEWESNGQPE
     421	NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGK



SEQ ID NO: 7 (Nucleotide sequence of the coding region of RhD2 LC)

Signal peptide:	1-57
Variable region:	58-388
CDR1:			133-159
CDR2:			211-219
CDR3:			328-357
Constant region:	389-705 (lambda)

	 M   A   W   A   L   L   F   L   T   L   L   T   Q   G   T   G   S   W   A   Q
       1	ATG GCC TGG GCT CTG CTA TTC CTC ACC CTC CTC ACT CAG GGC ACA GGG TCC TGG GCC CAG

	 S   A   L   T   Q   P   A   S   V   S   G   S   P   G   Q   S   I   T   I   S
      61	TCT GCC CTG ACT CAG CCT GCC TCC GTG TCT GGG TCT CCT GGA CAG TCG ATC ACC ATC TCC

	 C   S   G   S   S   S   D   V   G   A   Y   K   Y   V   S   W   Y   Q   Q   H
     121	TGC AGT GGA AGC AGC AGT GAC GTT GGT GCT TAT AAG TAT GTC TCC TGG TAC CAA CAA CAC

	 P   G   K   T   P   K   L   M   I   Y   D   V   N   N   R   P   S   G   V   S
     181	CCA GGC AAA ACC CCC AAA CTC ATG ATT TAT GAT GTC AAT AAT CGG CCC TCA GGG GTT TCT

	 D   R   F   S   G   S   K   S   G   N   T   A   F   L   T   I   S   G   L   Q
     241	GAT CGC TTC TCT GGC TCC AAG TCT GGC AAC ACG GCC TTT CTG ACC ATC TCT GGG CTC CAG

	 A   E   D   E   A   D   Y   Y   C   N   S   Y   T   S   S   S   T   R   V   F
     301	GCT GAG GAC GAG GCT GAT TAT TAC TGC AAC TCA TAT ACA AGC AGC AGC ACT CGA GTG TTC
	 G   G   G   T   K   L   T   V   L   G   Q   P   K   A   A   P   S   V   T   L
     361	GGC GGA GGG ACC AAG CTG ACC GTC CTA GGT CAG CCC AAG GCT GCC CCC TCG GTC ACT CTG

	 F   P   P   S   S   E   E   L   Q   A   N   K   A   T   L   V   C   L   I   S
     421	TTC CCA CCC TCC TCT GAG GAG CTT CAA GCC AAC AAG GCC ACA CTG GTG TGT CTC ATA AGT

	 D   F   Y   P   G   A   V   T   V   A   W   K   A   D   S   S   P   V   K   A
     481	GAC TTC TAC CCG GGA GCC GTG ACA GTG GCC TGG AAG GCA GAT AGC AGC CCC GTC AAG GCG

	 G   V   E   T   T   T   P   S   K   Q   S   N   N   K   Y   A   A   S   S   Y
     541	GGA GTG GAG ACC ACC ACA CCC TCC AAA CAA AGC AAC AAC AAG TAC GCG GCC AGC AGC TAC

	 L   S   L   T   P   E   Q   W   K   S   H   K   S   Y   S   C   Q   V   T   H
     601	CTG AGC CTG ACG CCT GAG CAG TGG AAG TCC CAC AAA AGC TAC AGC TGC CAG GTC ACG CAT

	 E   G   S   T   V   E   K   T   V   A   P   T   E   C   S   *
     661	GAA GGG AGC ACC GTG GAG AAG ACA GTG GCC CCT ACA GAA TGT TCA TAG



SEQ ID NO: 8 (RhD2 LC: amino acid sequence)

       1	MAWALLFLTL LTQGTGSWAQ SALTQPASVS GSPGQSITIS CSGSSSDVGA YKYVSWYQQH
      61	PGKTPKLMIY DVNNRPSGVS DRFSGSKSGN TAFLTISGLQ AEDEADYYCN SYTSSSTRVF
     121	GGGTKLTVLG QPKAAPSVTL FPPSSEELQA NKATLVCLIS DFYPGAVTVA WKADSSPVKA
     181	GVETTTPSKQ SNNKYAASSY LSLTPEQWKS HKSYSCQVTH EGSTVEKTVA PTECS



SEQ ID NO: 9 (Nucleotide sequence of the coding region of RhD3 HC)

Signal peptide:	1-57
Variable region:	58-448
CDR1:			133-162
CDR2:			214-234
CDR3:			349-414
Constant region:	449-1578 (gamma3)

	 M   D   T   L   C   Y   T   L   L   L   L   T   T   P   S   W   V   L   S   Q
       1	ATG GAC ACA CTT TGC TAC ACA CTC CTG CTG CTG ACC ACC CCT TCC TGG GTC TTG TCC CAG

	 V   T   L   K   E   S   G   P   V   L   V   K   P   T   E   T   L   T   L   T
      61	GTC ACC TTG AAG GAG TCT GGT CCT GTG CTG GTG AAA CCC ACA GAG ACC CTC ACG CTG ACC

	 C   T   V   S   G   F   S   L   N   N   A   R   M   G   V   S   W   I   R   Q
     121	TGC ACC GTC TCT GGG TTC TCA CTC AAC AAT GCT AGA ATG GGT GTG AGC TGG ATC CGT CAG

	 P   P   G   K   A   L   E   W   L   A   H   I   F   S   N   D   E   K   S   Y
     181	CCC CCA GGG AAG GCC CTG GAG TGG CTT GCA CAC ATT TTT TCG AAT GAC GAA AAA TCC TAC

	 S   T   S   L   K   S   R   L   T   I   S   K   D   T   S   K   S   Q   V   F
     241	AGC ACA TCT CTG AAG AGC AGG CTC ACC ATC TCC AAG GAC ACC TCC AAA AGC CAG GTG TTC

	 L   T   M   T   N   M   D   P   V   D   T   A   T   Y   Y   C   A   R   T   P
     301	CTT ACC ATG ACC AAC ATG GAC CCT GTG GAC ACA GCC ACA TAT TAC TGT GCA CGG ACC CCT

	 I   T   M   V   R   G   A   I   R   L   Y   Y   Y   Y   Y   M   D   V   W   G
     361	ATT ACT ATG GTT CGG GGA GCT ATT AGG CTA TAC TAC TAC TAC TAC ATG GAC GTC TGG GGC

	 K   G   T   T   V   T   V   S   S   A   S   T   K   G   P   S   V   F   P   L
     421	AAA GGG ACC ACG GTC ACC GTC TCC TCA GCT TCC ACC AAG GGC CCA TCG GTC TTC CCC CTG

	 A   P   C   S   R   S   T   S   G   G   T   A   A   L   G   C   L   V   K   D
     481	GCG CCC TGC TCC AGG AGC ACC TCT GGG GGC ACA GCG GCC CTG GGC TGC CTG GTC AAG GAC

	 Y   F   P   E   P   V   T   V   S   W   N   S   G   A   L   T   S   G   V   H
     541	TAC TTC CCC GAA CCG GTG ACG GTG TCG TGG AAC TCA GGC GCC CTG ACC AGC GGC GTG CAC

	 T   F   P   A   V   L   Q   S   S   G   L   Y   S   L   S   S   V   V   T   V
     601	ACC TTC CCG GCT GTC CTA CAG TCC TCA GGA CTC TAC TCC CTC AGC AGC GTG GTG ACC GTG

	 P   S   S   S   L   G   T   Q   T   Y   T   C   N   V   N   H   K   P   S   N
     661	CCC TCC AGC AGC TTG GGC ACC CAG ACC TAC ACC TGC AAC GTG AAT CAC AAG CCC AGC AAC

	 T   K   V   D   K   R   V   E   L   K   T   P   L   G   D   T   T   H   T   C
     721	ACC AAG GTG GAC AAG AGA GTT GAG CTC AAA ACC CCA CTT GGT GAC ACA ACT CAC ACA TGC

	 P   R   C   P   E   P   K   S   C   D   T   P   P   P   C   P   R   C   P   E
     781	CCA CGG TGC CCA GAG CCC AAA TCT TGT GAC ACA CCT CCC CCG TGC CCA CGG TGC CCA GAG

	 P   K   S   C   D   T   P   P   P   C   P   R   C   P   E   P   K   S   C   D
     841	CCC AAA TCT TGT GAC ACA CCT CCC CCA TGC CCA CGG TGC CCA GAG CCC AAA TCT TGT GAC

	 T   P   P   P   C   P   R   C   P   A   P   E   L   L   G   G   P   S   V   F
     901	ACA CCT CCC CCA TGC CCA CGG TGC CCA GCA CCT GAA CTC CTG GGA GGA CCG TCA GTC TTC

	 L   F   P   P   K   P   K   D   T   L   M   I   S   R   T   P   E   V   T   C
     961	CTC TTC CCC CCA AAA CCC AAG GAT ACC CTT ATG ATT TCC CGG ACC CCT GAG GTC ACG TGC

	 V   V   V   D   V   S   H   E   D   P   E   V   Q   F   K   W   Y   V   D   G
    1021	GTG GTG GTG GAC GTG AGC CAC GAA GAC CCC GAG GTC CAG TTC AAG TGG TAC GTG GAC GGC

	 V   E   V   H   N   A   K   T   K   P   R   E   E   Q   F   N   S   T   F   R
    1081	GTG GAG GTG CAT AAT GCC AAG ACA AAG CCG CGG GAG GAG CAG TTC AAC AGC ACG TTC CGT

	 V   V   S   V   L   T   V   L   H   Q   D   W   L   N   G   K   E   Y   K   C
    1141	GTG GTC AGC GTC CTC ACC GTC CTG CAC CAG GAC TGG CTG AAC GGC AAG GAG TAC AAG TGC


	 K   V   S   N   K   A   L   P   A   P   I   E   K   T   I   S   K   T   K   G
    1201	AAG GTC TCC AAC AAA GCC CTC CCA GCC CCC ATC GAG AAA ACC ATC TCC AAA ACC AAA GGA

	 Q   P   R   E   P   Q   V   Y   T   L   P   P   S   R   E   E   M   T   K   N
    1261	CAG CCC CGA GAA CCA CAG GTG TAC ACC CTG CCC CCA TCC CGG GAG GAG ATG ACC AAG AAC

	 Q   V   S   L   T   C   L   V   K   G   F   Y   P   S   D   I   A   V   E   W
    1321	CAG GTC AGC CTG ACC TGC CTG GTC AAA GGC TTC TAC CCC AGC GAC ATC GCC GTG GAG TGG

	 E   S   S   G   Q   P   E   N   N   Y   N   T   T   P   P   M   L   D   S   D
    1381	GAG AGC AGC GGG CAG CCG GAG AAC AAC TAC AAC ACC ACG CCT CCC ATG CTG GAC TCC GAC

	 G   S   F   F   L   Y   S   K   L   T   V   D   K   S   R   W   Q   Q   G   N
    1441	GGC TCC TTC TTC CTC TAC AGC AAG CTC ACC GTG GAC AAG AGC AGG TGG CAG CAG GGG AAC

	 I   F   S   C   S   V   M   H   E   A   L   H   N   R   F   T   Q   K   S   L
    1501	ATC TTC TCA TGC TCC GTG ATG CAT GAG GCT CTG CAC AAC CGC TTC ACG CAG AAG AGC CTC

	 S   L   S   P   G   K   *
    1561	TCC CTG TCT CCG GGT AAA TGA



SEQ ID NO: 10 (RhD3 HC: amino acid sequence)

       1	MDTLCYTLLL LTTPSWVLSQ VTLKESGPVL VKPTETLTLT CTVSGFSLNN ARMGVSWIRQ
      61	PPGKALEWLA HIFSNDEKSY STSLKSRLTI SKDTSKSQVF LTMTNMDPVD TATYYCARTP
     121	ITMVRGAIRL YYYYYMDVWG KGTTVTVSSA STKGPSVFPL APCSRSTSGG TAALGCLVKD
     181	YFPEPVTVSW NSGALTSGVH TFPAVLQSSG LYSLSSVVTV PSSSLGTQTY TCNVNHKPSN
     241	TKVDKRVELK TPLGDTTHTC PRCPEPKSCD TPPPCPRCPE PKSCDTPPPC PRCPEPKSCD
     301	TPPPCPRCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVQFKWYVDG
     361	VEVHNAKTKP REEQFNSTFR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKTKG
     421	QPREPQVYTL PPSREEMTKN QVSLTCLVKG FYPSDIAVEW ESSGQPENNY NTTPPMLDSD
     481	GSFFLYSKLT VDKSRWQQGN IFSCSVMHEA LHNRFTQKSL SLSPGK


SEQ ID NO: 11 (Nucleotide sequence of the coding region of RhD3 LC)

Signal peptide:	1-66
Variable region:	67-391
CDR1:			145-162
CDR2:			214-222
CDR3:			331-360 (kappa)
Constant region:	392-711

	 M   D   M   R   V   P   A   Q   L   L   G   L   L   L   L   W   L   R   G   A
       1	ATG GAC ATG AGG GTC CCC GCT CAG CTC CTG GGG CTC CTG CTA CTC TGG CTC CGA GGT GCC

	 R   C   D   I   Q   V   T   Q   S   P   S   S   L   S   A   S   V   G   D   R
      61	AGA TGT GAC ATC CAG GTG ACC CAG TCT CCG TCC TCC CTG TCT GCG TCT GTA GGA GAC AGA

	 V   T   I   N   C   R   A   S   Q   S   I   G   T   Y   L   N   W   Y   Q   Q
     121	GTC ACC ATC AAT TGC CGG GCA AGT CAG AGC ATT GGC ACC TAT TTA AAT TGG TAT CAG CAG

	 K   P   G   K   A   P   N   L   L   I   Y   A   A   S   S   L   Q   S   G   V
     181	AAA CCA GGG AAA GCC CCT AAC CTC CTG ATC TAT GCT GCA TCC AGT TTG CAG AGT GGG GTC

	 P   S   R   F   S   G   S   G   S   G   T   D   F   T   L   T   I   S   S   L
     241	CCA TCA AGG TTC AGT GGC AGT GGA TCT GGG ACA GAT TTC ACT CTC ACC ATC AGC AGT CTG

	 Q   P   E   D   F   A   T   Y   Y   C   Q   Q   T   Y   S   T   P   T   W   T
     301	CAA CCT GAA GAT TTT GCA ACT TAT TAC TGT CAA CAG ACT TAC AGT ACC CCC ACG TGG ACG

	 F   G   R   G   T   K   V   E   I   K   R   T   V   A   A   P   S   V   F   I
     361	TTC GGC CGA GGG ACC AAG GTG GAA ATC AAG CGA ACT GTG GCT GCA CCA TCT GTC TTC ATC

	 F   P   P   S   D   E   Q   L   K   S   G   T   A   S   V   V   C   L   L   N
     421	TTC CCG CCA TCT GAT GAG CAG TTG AAA TCT GGA ACT GCC TCT GTT GTG TGC CTG CTG AAT

	 N   F   Y   P   R   E   A   K   V   Q   W   K   V   D   N   A   L   Q   S   G
     481	AAC TTC TAT CCC AGA GAG GCC AAA GTA CAG TGG AAG GTG GAT AAC GCC CTC CAA TCG GGT

	 N   S   Q   E   S   V   T   E   Q   D   S   K   D   S   T   Y   S   L   S   S
     541	AAC TCC CAG GAG AGT GTC ACA GAG CAG GAC AGC AAG GAC AGC ACC TAC AGC CTC AGC AGC

	 T   L   T   L   S   K   A   D   Y   E   K   H   K   L   Y   A   C   E   V   T
     601	ACC CTG ACG CTG AGC AAA GCA GAC TAC GAG AAA CAC AAA CTC TAC GCC TGC GAA GTC ACC

	 H   Q   G   L   S   S   P   V   T   K   S   F   N   R   G   E   C   *
     661	CAT CAG GGC CTG AGC TCG CCC GTC ACA AAG AGC TTC AAC AGG GGA GAG TGT TAG



SEQ ID NO: 12 (RhD3 LC: amino acid sequence)

       1	MDMRVPAQLL GLLLLWLRGA RCDIQVTQSP SSLSASVGDR VTINCRASQS IGTYLNWYQQ
      61	KPGKAPNLLI YAASSLQSGV PSRFSGSGSG TDFTLTISSL QPEDFATYYC QQTYSTPTWT
     121	FGRGTKVEIK RTVAAPSVFI FPPSDEQLKS GTASVVCLLN NFYPREAKVQ WKVDNALQSG
     181	NSQESVTEQD SKDSTYSLSS TLTLSKADYE KHKLYACEVT HQGLSSPVTK SFNRGEC



SEQ ID NO:13 (PCR forward primer)

5-GACTGAATTCTTTTTTTTTTTTTTTTTTTTV-3



SEQ ID NO: 14 (PCR gamma chain reverse primer)

5-ACTGGAATTCGGTGCTTTATTTCCATGCTGG-3



SEQ ID NO: 15 (PCR gamma chain reverse primer)

5-ACTGGAATTCGTACGTGCCAAGCATCCTCG-3
SEQ ID NO: 16 (PCR kappa chain reverse primer)

5-ACTGGAATTCAGAGGCCAAAGGATGGGAGG-3



SEQ ID NO: 17 (PCR kappa chain reverse primer)

5-GACTGAATTCCTGGAACTGAGGAGCAGGTGG-3



SEQ ID NO: 18 (PCR lambda chain reverse primer)

5-GACTGAATTCCCTGGGATCCTGCAGCTC-3



SEQ ID NO: 19 (PCR lambda chain reverse primer)

5-ACTGGAATTCGGGGTGAGGGTTGAGAACC-3



SEQ ID NO: 20 (RhD1 HC forward gene-specific primer)

5-ATCGTCTAGAGCCACCATGGACTGGACCTGGAGGTTCC-3



SEQ ID NO: 21 (RhD2 HC Forward gene-specific primer)

5-ATCGTCTAGAGCCACCATGGACTGGACCTGGAGGTTCC-3



SEQ ID NO: 22 (RhD3 HC Forward gene-specific primer)

5-ATCGTCTAGAGCCACCATGGACACACTTTGCTACACACTCC-3



SEQ ID NO: 23 (Reverse primer used for all heavy chains)

5-TGACGAATTCCACTCATTTACCCGGAGACAGG-3



SEQ ID NO: 24 (RhD1 and RhD2 LC Forward gene-specific primer)

5-ATCGTCTAGAGCCACCATGGCCTGGGCTCTGCTATTC-3
SEQ ID NO: 25 (RhD1 and RhD2 LC Reverse primer)

5-ACTGGAATTCGAACCTATGAACATTCTGTAGGGG-3



SEQ ID NO: 26 (RhD3 LC Forward gene-specific primer)

5-ATCGTCTAGAGCCACCATGGACATGAGGGTCCCCG-3



SEQ ID NO: 27 (RhD3 LC Reverse primer)

5-GACTGAATTCCTAACACTCTCCCCTGTTGAAGC-3



SEQ ID NO: 28 (forward primer for amplification of RhD3 constant region)

5-ATCGTCTAGAGTCAGCTAGCACCAAGGGCCCATCGGTCTTCC-3



SEQ ID NO: 29 (reverse primer for amplification of RhD3 constant region)

5-TGACGAATTCCACTCATTTACCCGGAGACAGG-3



SEQ ID NO: 30 (forward primer for amplification of RhD1 variable domain)
 
5-ATCGTCTAGAGCCACCATGGACTGGACCTGGAGGTTCC-3



SEQ ID NO: 31 (reverse primer for amplification of RhD1 variable domain)

5-GATGCTAGCTGAGGAGACGGTGATCGTGG-3



SEQ ID NO: 32 (Sequence of RhD1 HC insert for pCB3 expression vector)

	XbaI  Kozak  Start
       1	TCTAGAGCCA CCATGGACTG GACCTGGAGG TTCCTCTTTG TGGTGGCAGC AGCTACAGGT

      61	GTCCAGTCCC AGGTGCAGCT GGTGCAGTCT GGGGCTGAGG TGAAGAAGCC TGGGTCCTCG

     121	GTGAAGGTCT CCTGCAAGGC TTCTGGAGGC ATCTTCAGAA CCTATGCTAT CAGCTGGGTG

     181	CGACAGGCCC CTGGACAAGG GCTTGAGTGG ATGGGAGGGA TCATCCCTAT GTTTGGTACA

     241	GTAAACTACG CACAGAAGTT CCAGGGCAGA GTCACGATTA GCGCGGACAA ATCCACGAGC

     301	ACAGCCTATA TGGAACTGAG CAGACTGAGA TCTGAGGACA CGGCCGTGTA TTACTGTGCG

     361	AGGCCGCCTT CCGGGGGTTG TGGTGGTGAC TGCTCACGGA GGGGCTACTA CTACGCCATG

     421	GACGTCTGGG GCCAAGGGAC CACGATCACC GTCTCCTCAG CCTCCACCAA GGGCCCATCG

     481	GTCTTCCCCC TGGCACCCTC CTCCAAGAGC ACCTCTGGGG GCACAGCGGC CCTGGGCTGC

     541	CTGGTCAAGG ACTACTTCCC CGAACCGGTG ACGGTGTCGT GGAACTCAGG CGCCCTGACC

     601	AGCGGCGTGC ACACCTTCCC GGCTGTCCTA CAGTCCTCAG GACTCTACTC CCTCAGCAGC

     661	GTGGTGACCG TGCCCTCCAG CAGCTTGGGC ACCCAGACCT ACATCTGCAA CGTGAATCAC

     721	AAGCCCAGCA ACACCAAGGT GGACAAGAAA GTTGAGCCCA AATCTTGTGA CAAAACTCAC

     781	ACATGCCCAC CGTGCCCAGC ACCTGAACTC CTGGGGGGAC CGTCAGTCTT CCTCTTCCCC

     841	CCAAAACCCA AGGACACCCT CATGATCTCC CGGACCCCTG AGGTCACATG CGTGGTGGTG

     901	GACGTGAGCC ACGAAGACCC TGAGGTCAAG TTCAACTGGT ACGTGGACGG CGTGGAGGTG

     961	CATAATGCCA AGACAAAGCC GCGGGAGGAG CAGTACAACA GCACGTACCG TGTGGTCAGC

    1021	GTCCTCACCG TCCTGCACCA GGACTGGCTG AATGGCAAGG AGTACAAGTG CAAGGTCTCC

    1081	AACAAAGCCC TCCCAGCCCC CATCGAGAAA ACCATCTCCA AAGCCAAAGG GCAGCCCCGA

    1141	GAACCACAGG TGTACACCCT GCCCCCATCC CGGGATGAGC TGACCAAGAA CCAGGTCAGC

    1201	CTGACCTGCC TGGTCAAAGG CTTCTATCCC AGCGACATCG CCGTGGAGTG GGAGAGCAAT

    1261	GGGCAGCCGG AGAACAACTA CAAGACCACG CCTCCCGTGC TGGACTCCGA CGGCTCCTTC

    1321	TTCCTCTACA GCAAGCTCAC CGTGGACAAG AGCAGGTGGC AGCAGGGGAA CGTCTTCTCA

    1381	TGCTCCGTGA TGCATGAGGC TCTGCACAAC CACTACACGC AGAAGAGCCT CTCCCTGTCT
	         Stop   EcoRI
    1441	CCGGGTAAAT GAGTGGAATT C



SEQ ID NO: 33 (Sequence of RhD1 LC insert for pCB11 expression vector)

	XbaI  Kozak  Start
       1	TCTAGAGCCA CCATGGCCTG GGCTCTGCTA TTCCTCACCC TCCTCACTCA GGGCACAGGG

      61	TCCTGGGCCC AGTCTGCCCT GACTCAACCT GCCTCCGTGT CTGGGTCTCC TGGACAGTCG

     121	ATCACCATCT CCTGCAGTGG AAGCAGCAGT GACGTTGGTG GTTATAAGTA TGTCTCCTGG

     181	TACCAACAAC ACCCAGGCAA AGCCCCCCAA CTCATGATTT ATGATGTCAA TAATCGGCCC

     241	TCAGGGGTTT CTAATCGCTT CTCTGGCTCC AAGTCTGGCA ACACGGCCTC CCTGACCATC


     301	TCTGGGCTCC AGGCTGAGGA CGAGGCTGAT TATTACTGCA GCTCATATAC AAGCAGCAGC

     361	ACTCGAGTGT TCGGCGGAGG GACGAAGCTG ACCGTCCTAG GTCAGCCCAA GGCTGCCCCC

     421	TCGGTCACTC TGTTCCCACC CTCCTCTGAG GAGCTTCAAG CCAACAAGGC CACACTGGTG

     481	TGTCTCATAA GTGACTTCTA CCCGGGAGCC GTGACAGTGG CCTGGAAGGC AGATAGCAGC

     541	CCCGTCAAGG CGGGAGTGGA GACCACCACA CCCTCCAAAC AAAGCAACAA CAAGTACGCG

     601	GCCAGCAGCT ACCTGAGCCT GACGCCTGAG CAGTGGAAGT CCCACAAAAG CTACAGCTGC
                                                                       Stop
     661	CAGGTCACGC ATGAAGGGAG CACCGTGGAG AAGACAGTGG CCCCTACAGA ATGTTCATAG
	    EcoRI
     721	GTTCGAATTC



SEQ ID NO: 34 (Sequence of RhD2 HC insert for pCB3 expression vector)

	XbaI  Kozak  Start
       1	TCTAGAGCCA CCATGGACTG GACCTGGAGG TTCCTCTTTG TGGTGGCAGC AGCTACAGGT

      61	GTCCAGTCCC AGGTGCAGCT GGTGCAGTCT GGGGCTGAGG TGAAGAAGCC TGGGTCCTCG

     121	GTGAAGGTCT CCTGCAAACC TTCTGGAGGC ATCTTCAGCA CCTATGCTAT CAGCTGGGTG

     181	CGACAGGCCC CGGGACAAGG GCTTGAGTGG ATGGGAGGGA TCATCCCTAT GTTTGGGACA

     241	GTAAACTACG CACAGAAGTT CCAGGGCAGA GTCACCATTA GCGCGGGCAA ATCCACGAGC

     301	ACAGCCGATA TGGAACTGAG CAGACTGAGA TCTGAGGACA CGGCCGTGTA TTACTGTGCG

     361	AGGCCGCCTT CCGGGGGTTG TGGTGGTGAC TGCTCACGGA GGGGCTATTA TTATGGTATG

     421	GACGTCTGGG GCCAAGGGAC CACGGTCATC GTCTCCTCAG CCTCCACCAA GGGCCCATCG

     481	GTCTTCCCCC TGGCACCCTC CTCCAAGAGC ACCTCTGGGG GCACAGCGGC CCTGGGCTGC

     541	CTGGTCAAGG ACTACTTCCC CGAACCGGTG ACGGTGTCGT GGAACTCAGG CGCCCTGACC

     601	AGCGGCGTGC ACACCTTCCC GGCTGTCCTA CAGTCCTCAG GACTCTACTC CCTCAGCAGC

     661	GTGGTGACCG TGCCCTCCAG CAGCTTGGGC ACCCAGACCT ACATCTGCAA CGTGAATCAC

     721	AAGCCCAGCA ACACCAAGGT GGACAAGAAA GTTGAGCCCA AATCTTGTGA CAAAACTCAC

     781	ACATGCCCAC CGTGCCCAGC ACCTGAACTC CTGGGGGGAC CGTCAGTCTT CCTCTTCCCC

     841	CCAAAACCCA AGGACACCCT CATGATCTCC CGGACCCCTG AGGTCACATG CGTGGTGGTG

     901	GACGTGAGCC ACGAAGACCC TGAGGTCAAG TTCAACTGGT ACGTGGACGG CGTGGAGGTG

     961	CATAATGCCA AGACAAAGCC GCGGGAGGAG CAGTACAACA GCACGTACCG TGTGGTCAGC

    1021	GTCCTCACCG TCCTGCACCA GGACTGGCTG AATGGCAAGG AGTACAAGTG CAAGGTCTCC

    1081	AACAAAGCCC TCCCAGCCCC CATCGAGAAA ACCATCTCCA AAGCCAAAGG GCAGCCCCGA

    1141	GAACCACAGG TGTACACCCT GCCCCCATCC CGGGATGAGC TGACCAAGAA CCAGGTCAGC

    1201	CTGACCTGCC TGGTCAAAGG CTTCTATCCC AGCGACATCG CCGTGGAGTG GGAGAGCAAT

    1261	GGGCAGCCGG AGAACAACTA CAAGACCACG CCTCCCGTGC TGGACTCCGA CGGCTCCTTC

    1321	TTCCTCTACA GCAAGCTCAC CGTGGACAAG AGCAGGTGGC AGCAGGGGAA CGTCTTCTCA

    1381	TGCTCCGTGA TGCATGAGGC TCTGCACAAC CACTACACGC AGAAGAGCCT CTCCCTGTCT
	         Stop   EcoRI
    1441	CCGGGTAAAT GAGTGGAATT C


SEQ ID NO: 35 (Sequence of RhD2 LC insert for pCB11 expression vector)

	XbaI  Kozak  Start
       1	TCTAGAGCCA CCATGGCCTG GGCTCTGCTA TTCCTCACCC TCCTCACTCA GGGCACAGGG

      61	TCCTGGGCCC AGTCTGCCCT GACTCAGCCT GCCTCCGTGT CTGGGTCTCC TGGACAGTCG

     121	ATCACCATCT CCTGCAGTGG AAGCAGCAGT GACGTTGGTG CTTATAAGTA TGTCTCCTGG

     181	TACCAACAAC ACCCAGGCAA AACCCCCAAA CTCATGATTT ATGATGTCAA TAATCGGCCC

     241	TCAGGGGTTT CTGATCGCTT CTCTGGCTCC AAGTCTGGCA ACACGGCCTT TCTGACCATC

     301	TCTGGGCTCC AGGCTGAGGA CGAGGCTGAT TATTACTGCA ACTCATATAC AAGCAGCAGC

     361	ACTCGAGTGT TCGGCGGAGG GACCAAGCTG ACCGTCCTAG GTCAGCCCAA GGCTGCCCCC

     421	TCGGTCACTC TGTTCCCACC CTCCTCTGAG GAGCTTCAAG CCAACAAGGC CACACTGGTG

     481	TGTCTCATAA GTGACTTCTA CCCGGGAGCC GTGACAGTGG CCTGGAAGGC AGATAGCAGC

     541	CCCGTCAAGG CGGGAGTGGA GACCACCACA CCCTCCAAAC AAAGCAACAA CAAGTACGCG

     601	GCCAGCAGCT ACCTGAGCCT GACGCCTGAG CAGTGGAAGT CCCACAAAAG CTACAGCTGC
                                                                       Stop
     661	CAGGTCACGC ATGAAGGGAG CACCGTGGAG AAGACAGTGG CCCCTACAGA ATGTTCATAG
	    EcoRI
     721	GTTCGAATTC



SEQ ID NO: 36 (Sequence of RhD3 HC insert for pCB3 expression vector)

	XbaI  Kozak  Start
       1	TCTAGAGCCA CCATGGACAC ACTTTGCTAC ACACTCCTGC TGCTGACCAC CCCTTCCTGG

      61	GTCTTGTCCC AGGTCACCTT GAAGGAGTCT GGTCCTGTGC TGGTGAAACC CACAGAGACC

     121	CTCACGCTGA CCTGCACCGT CTCTGGGTTC TCACTCAACA ATGCTAGAAT GGGTGTGAGC

     181	TGGATCCGTC AGCCCCCAGG GAAGGCCCTG GAGTGGCTTG CACACATTTT TTCGAATGAC

     241	GAAAAATCCT ACAGCACATC TCTGAAGAGC AGGCTCACCA TCTCCAAGGA CACCTCCAAA

     301	AGCCAGGTGT TCCTTACCAT GACCAACATG GACCCTGTGG ACACAGCCAC ATATTACTGT

     361	GCACGGACCC CTATTACTAT GGTTCGGGGA GCTATTAGGC TATACTACTA CTACTACATG

     421	GACGTCTGGG GCAAAGGGAC CACGGTCACC GTCTCCTCAG CTTCCACCAA GGGCCCATCG

     481	GTCTTCCCCC TGGCGCCCTG CTCCAGGAGC ACCTCTGGGG GCACAGCGGC CCTGGGCTGC

     541	CTGGTCAAGG ACTACTTCCC CGAACCGGTG ACGGTGTCGT GGAACTCAGG CGCCCTGACC

     601	AGCGGCGTGC ACACCTTCCC GGCTGTCCTA CAGTCCTCAG GACTCTACTC CCTCAGCAGC

     661	GTGGTGACCG TGCCCTCCAG CAGCTTGGGC ACCCAGACCT ACACCTGCAA CGTGAATCAC

     721	AAGCCCAGCA ACACCAAGGT GGACAAGAGA GTTGAGCTCA AAACCCCACT TGGTGACACA

     781	ACTCACACAT GCCCACGGTG CCCAGAGCCC AAATCTTGTG ACACACCTCC CCCGTGCCCA

     841	CGGTGCCCAG AGCCCAAATC TTGTGACACA CCTCCCCCAT GCCCACGGTG CCCAGAGCCC

     901	AAATCTTGTG ACACACCTCC CCCATGCCCA CGGTGCCCAG CACCTGAACT CCTGGGAGGA

     961	CCGTCAGTCT TCCTCTTCCC CCCAAAACCC AAGGATACCC TTATGATTTC CCGGACCCCT

    1021	GAGGTCACGT GCGTGGTGGT GGACGTGAGC CACGAAGACC CCGAGGTCCA GTTCAAGTGG

    1081	TACGTGGACG GCGTGGAGGT GCATAATGCC AAGACAAAGC CGCGGGAGGA GCAGTTCAAC

    1141	AGCACGTTCC GTGTGGTCAG CGTCCTCACC GTCCTGCACC AGGACTGGCT GAACGGCAAG

    1201	GAGTACAAGT GCAAGGTCTC CAACAAAGCC CTCCCAGCCC CCATCGAGAA AACCATCTCC

    1261	AAAACCAAAG GACAGCCCCG AGAACCACAG GTGTACACCC TGCCCCCATC CCGGGAGGAG

    1321	ATGACCAAGA ACCAGGTCAG CCTGACCTGC CTGGTCAAAG GCTTCTACCC CAGCGACATC

    1381	GCCGTGGAGT GGGAGAGCAG CGGGCAGCCG GAGAACAACT ACAACACCAC GCCTCCCATG

    1441	CTGGACTCCG ACGGCTCCTT CTTCCTCTAC AGCAAGCTCA CCGTGGACAA GAGCAGGTGG

    1501	CAGCAGGGGA ACATCTTCTC ATGCTCCGTG ATGCATGAGG CTCTGCACAA CCGCTTCACG
	                                 Stop  EcoRI
    1561	CAGAAGAGCC TCTCCCTGTC TCCGGGTAAA TGAGTGGAAT TC



SEQ ID NO: 37 (Sequence of RhD3 LC insert for pCB11 expression vector)

	XbaI  Kozak  Start
       1	TCTAGAGCCA CCATGGACAT GAGGGTCCCC GCTCAGCTCC TGGGGCTCCT GCTACTCTGG

      61	CTCCGAGGTG CCAGATGTGA CATCCAGGTG ACCCAGTCTC CGTCCTCCCT GTCTGCGTCT

     121	GTAGGAGACA GAGTCACCAT CAATTGCCGG GCAAGTCAGA GCATTGGCAC CTATTTAAAT

     181	TGGTATCAGC AGAAACCAGG GAAAGCCCCT AACCTCCTGA TCTATGCTGC ATCCAGTTTG

     241	CAGAGTGGGG TCCCATCAAG GTTCAGTGGC AGTGGATCTG GGACAGATTT CACTCTCACC

     301	ATCAGCAGTC TGCAACCTGA AGATTTTGCA ACTTATTACT GTCAACAGAC TTACAGTACC

     361	CCCACGTGGA CGTTCGGCCG AGGGACCAAG GTGGAAATCA AGCGAACTGT GGCTGCACCA

     421	TCTGTCTTCA TCTTCCCGCC ATCTGATGAG CAGTTGAAAT CTGGAACTGC CTCTGTTGTG

     481	TGCCTGCTGA ATAACTTCTA TCCCAGAGAG GCCAAAGTAC AGTGGAAGGT GGATAACGCC

     541	CTCCAATCGG GTAACTCCCA GGAGAGTGTC ACAGAGCAGG ACAGCAAGGA CAGCACCTAC

     601	AGCCTCAGCA GCACCCTGAC GCTGAGCAAA GCAGACTACG AGAAACACAA ACTCTACGCC

     661	TGCGAAGTCA CCCATCAGGG CCTGAGCTCG CCCGTCACAA AGAGCTTCAA CAGGGGAGAG
	  StopEcoRI
     721	TGTTAGGAAT TC



SEQ ID NO: 38 (Sequence of RhD1V3C HC insert for pCB3 expression vector)

	XbaI  Kozak  Start
       1	TCTAGAGCCA CCATGGACTG GACCTGGAGG TTCCTCTTTG TGGTGGCAGC AGCTACAGGT

      61	GTCCAGTCCC AGGTGCAGCT GGTGCAGTCT GGGGCTGAGG TGAAGAAGCC TGGGTCCTCG

     121	GTGAAGGTCT CCTGCAAGGC TTCTGGAGGC ATCTTCAGAA CCTATGCTAT CAGCTGGGTG

     181	CGACAGGCCC CTGGACAAGG GCTTGAGTGG ATGGGAGGGA TCATCCCTAT GTTTGGTACA

     241	GTAAACTACG CACAGAAGTT CCAGGGCAGA GTCACGATTA GCGCGGACAA ATCCACGAGC

     301	ACAGCCTATA TGGAACTGAG CAGACTGAGA TCTGAGGACA CGGCCGTGTA TTACTGTGCG

     361	AGGCCGCCTT CCGGGGGTTG TGGTGGTGAC TGCTCACGGA GGGGCTACTA CTACGCCATG
	                                          NheI
     421	GACGTCTGGG GCCAAGGGAC CACGATCACC GTCTCCTCAG CTAGCACCAA GGGCCCATCG

     481	GTCTTCCCCC TGGCGCCCTG CTCCAGGAGC ACCTCTGGGG GCACAGCGGC CCTGGGCTGC

     541	CTGGTCAAGG ACTACTTCCC CGAACCGGTG ACGGTGTCGT GGAACTCAGG CGCCCTGACC

     601	AGCGGCGTGC ACACCTTCCC GGCTGTCCTA CAGTCCTCAG GACTCTACTC CCTCAGCAGC

     661	GTGGTGACCG TGCCCTCCAG CAGCTTGGGC ACCCAGACCT ACACCTGCAA CGTGAATCAC

     721	AAGCCCAGCA ACACCAAGGT GGACAAGAGA GTTGAGCTCA AAACCCCACT TGGTGACACA

     781	ACTCACACAT GCCCACGGTG CCCAGAGCCC AAATCTTGTG ACACACCTCC CCCGTGCCCA

     841	CGGTGCCCAG AGCCCAAATC TTGTGACACA CCTCCCCCAT GCCCACGGTG CCCAGAGCCC

     901	AAATCTTGTG ACACACCTCC CCCATGCCCA CGGTGCCCAG CACCTGAACT CCTGGGAGGA

     961	CCGTCAGTCT TCCTCTTCCC CCCAAAACCC AAGGATACCC TTATGATTTC CCGGACCCCT

    1021	GAGGTCACGT GCGTGGTGGT GGACGTGAGC CACGAAGACC CCGAGGTCCA GTTCAAGTGG

    1081	TACGTGGACG GCGTGGAGGT GCATAATGCC AAGACAAAGC CGCGGGAGGA GCAGTTCAAC

    1141	AGCACGTTCC GTGTGGTCAG CGTCCTCACC GTCCTGCACC AGGACTGGCT GAACGGCAAG

    1201	GAGTACAAGT GCAAGGTCTC CAACAAAGCC CTCCCAGCCC CCATCGAGAA AACCATCTCC

    1261	AAAACCAAAG GACAGCCCCG AGAACCACAG GTGTACACCC TGCCCCCATC CCGGGAGGAG

    1321	ATGACCAAGA ACCAGGTCAG CCTGACCTGC CTGGTCAAAG GCTTCTACCC CAGCGACATC

    1381	GCCGTGGAGT GGGAGAGCAG CGGGCAGCCG GAGAACAACT ACAACACCAC GCCTCCCATG

    1441	CTGGACTCCG ACGGCTCCTT CTTCCTCTAC AGCAAGCTCA CCGTGGACAA GAGCAGGTGG

    1501	CAGCAGGGGA ACATCTTCTC ATGCTCCGTG ATGCATGAGG CTCTGCACAA CCGCTTCACG
	                                 Stop  EcoRI
    1561	CAGAAGAGCC TCTCCCTGTC TCCGGGTAAA TGAGTGGAAT TC


SEQ ID NO: 39 (Nucleotide sequence of optimized RhD1 HC coding region)

	BamHI Kozak  Start
       1	GGATCCGCCA CCATGGACTG GACCTGGCGC TTCCTGTTCG TGGTGGCCGC CGCCACCGGC
      61	GTGCAGAGCC AGGTGCAGCT GGTGCAGAGC GGCGCCGAGG TGAAGAAGCC CGGCAGCAGC
     121	GTCAAGGTGT CCTGCAAGGC CAGCGGCGGC ATCTTCCGCA CCTACGCCAT CTCTTGGGTC
     181	CGGCAGGCTC CCGGGCAGGG GCTCGAGTGG ATGGGCGGCA TCATCCCCAT GTTCGGCACC
     241	GTGAACTACG CCCAGAAGTT CCAGGGCCGC GTCACCATCA GCGCCGACAA GAGCACCAGC
     301	ACCGCCTACA TGGAGCTGTC CCGCCTGCGC TCCGAGGACA CCGCCGTGTA CTACTGTGCT
     361	AGGCCTCCCA GCGGCGGCTG CGGCGGCGAC TGCAGCCGCA GGGGCTACTA CTACGCTATG
     421	GACGTGTGGG GCCAGGGCAC CACCATCACC GTGAGCAGCG CTAGCACCAA GGGCCCCAGC
     481	GTGTTCCCCC TGGCCCCCAG CAGCAAGAGC ACCTCCGGCG GCACCGCCGC CCTGGGCTGC
     541	CTGGTGAAGG ACTACTTCCC CGAGCCCGTG ACCGTGAGCT GGAACAGCGG CGCCCTGACC
     601	AGCGGCGTGC ACACCTTCCC CGCCGTGCTG CAGAGCAGCG GCCTGTACAG CCTGAGCAGC
     661	GTGGTGACCG TGCCCAGCAG CAGCCTGGGC ACCCAGACCT ACATCTGCAA CGTGAACCAC
     721	AAGCCCAGCA ACACCAAGGT GGACAAGAAG GTGGAGCCCA AGAGCTGCGA CAAGACCCAC
     781	ACCTGCCCCC CCTGCCCCGC CCCCGAGCTG CTGGGCGGCC CCTCCGTGTT CCTGTTCCCC
     841	CCCAAGCCCA AGGACACCCT GATGATCAGC CGCACCCCCG AGGTGACCTG CGTGGTGGTG
     901	GACGTGAGCC ACGAGGACCC CGAGGTGAAG TTCAACTGGT ACGTGGACGG CGTGGAGGTG
     961	CACAACGCCA AGACCAAGCC CCGCGAGGAG CAGTACAACA GCACCTACCG CGTGGTGTCC
    1021	GTGCTGACCG TGCTGCACCA GGACTGGCTG AACGGCAAGG AGTACAAGTG CAAGGTCTCC
    1081	AACAAGGCCC TGCCCGCCCC CATCGAGAAG ACCATCAGCA AGGCCAAGGG GCAGCCTAGA
    1141	GAGCCCCAGG TCTACACCCT GCCTCCATCC CGCGACGAGC TGACCAAGAA CCAGGTGTCC
    1201	CTGACCTGTC TGGTCAAGGG CTTCTACCCC AGCGACATCG CCGTGGAGTG GGAGAGCAAC
    1261	GGCCAGCCCG AGAACAACTA CAAGACCACC CCCCCCGTGC TGGACAGCGA CGGCAGCTTC
    1321	TTCCTGTACA GCAAGCTGAC CGTGGACAAG AGCCGCTGGC AGCAGGGCAA CGTGTTCAGC
    1381	TGCAGCGTGA TGCACGAGGC CCTGCACAAC CACTACACCC AGAAGAGCCT GAGCCTGTCC
	         Stop   NotI
    1441	CCCGGCAAGT GATGAGCGGC CGC

SEQ ID NO: 40 (Nucleotide sequence of optimized RhD1 LC coding region)

	BamHI Kozak  Start
       1	GGATCCGCCA CCATGGCCTG GGCCCTGCTG TTCCTGACCC TGCTGACCCA GGGCACCGGC
      61	AGCTGGGCCC AGAGCGCCCT GACCCAGCCC GCCAGCGTGA GCGGCAGCCC CGGCCAGTCT
     121	ATCACCATCT CTTGTAGCGG CAGCAGCAGC GACGTGGGCG GCTACAAGTA CGTGTCTTGG
     181	TATCAGCAGC ACCCCGGCAA GGCCCCCCAG CTGATGATCT ACGACGTGAA CAACCGCCCC
     241	AGCGGCGTGA GCAACCGCTT CAGCGGCTCC AAGAGCGGCA ACACCGCCAG CCTGACCATC
     301	TCTGGGCTGC AGGCTGAGGA CGAGGCCGAC TACTACTGCA GCAGCTACAC CAGCAGCTCC
     361	ACCCGCGTGT TCGGCGGCGG CACCAAGCTG ACCGTGCTGG GCCAGCCCAA GGCCGCCCCC
     421	AGCGTGACCC TGTTCCCCCC CAGCAGCGAG GAGCTCCAGG CCAACAAGGC TACCCTGGTG
     481	TGCCTGATCA GCGACTTCTA CCCCGGCGCC GTGACCGTCG CCTGGAAGGC CGACAGCAGC
     541	CCCGTGAAGG CCGGCGTGGA GACCACCACC CCCAGCAAGC AGAGCAACAA CAAGTACGCC
     601	GCCAGCAGCT ACCTGAGCCT GACCCCCGAG CAGTGGAAGA GCCACAAGAG CTACAGCTGC
	                                                              Stop
     661	CAGGTGACCC ACGAGGGCAG CACCGTGGAG AAGACCGTGG CCCCCACCGA GTGCAGCTGA
	   NotI
     721	TGAGCGGCCG C


SEQ ID NO: 41 (Nucleotide sequence of optimized RhD3 HC coding region)

	BamHI Kozak  Start
       1	GGATCCGCCA CCATGGACAC CCTGTGCTAC ACCCTGCTGC TGCTGACCAC CCCCAGCTGG
      61	GTGCTGTCCC AGGTGACCCT GAAGGAGAGC GGCCCTGTCC TGGTGAAGCC CACCGAGACC
     121	CTGACCCTGA CCTGCACCGT GAGCGGCTTC AGCCTGAACA ACGCCCGCAT GGGCGTGAGC
     181	TGGATCAGGC AGCCCCCTGG CAAGGCCCTG GAGTGGCTGG CCCACATCTT CAGCAACGAC
     241	GAGAAGAGCT ACAGCACCAG CCTGAAGAGC CGCCTGACCA TCAGCAAGGA CACCAGCAAG
     301	AGCCAGGTGT TCCTGACCAT GACCAACATG GACCCCGTGG ACACCGCCAC CTACTACTGC
     361	GCCCGCACCC CCATCACAAT GGTCAGAGGC GCCATCCGCC TGTACTACTA CTATTACATG
     421	GACGTGTGGG GCAAGGGCAC CACCGTGACC GTGAGCAGCG CTAGCACCAA GGGCCCCAGC
     481	GTGTTCCCCC TGGCCCCCTG CAGCCGCAGC ACCTCTGGCG GCACCGCCGC TCTGGGCTGC
     541	CTGGTGAAGG ACTACTTCCC CGAGCCTGTG ACCGTGTCCT GGAACTCTGG CGCCCTGACC
     601	AGCGGCGTGC ACACCTTCCC CGCCGTGCTG CAGAGCAGCG GCCTGTACAG CCTGAGCAGC
     661	GTGGTGACCG TGCCCAGCAG CAGCCTGGGC ACCCAGACCT ACACCTGCAA CGTGAACCAC
     721	AAGCCCAGCA ACACCAAGGT GGACAAGCGC GTGGAGCTGA AGACCCCCCT GGGCGACACC
     781	ACCCACACCT GCCCCAGATG TCCCGAGCCC AAGAGCTGCG ACACCCCCCC TCCCTGCCCT
     841	CGCTGCCCTG AGCCTAAGTC CTGTGACACC CCTCCCCCTT GCCCCCGGTG TCCAGAGCCA
     901	AAGTCTTGCG ATACCCCACC CCCCTGTCCA AGGTGCCCTG CCCCCGAGCT GCTGGGCGGA
     961	CCCTCCGTGT TCCTGTTCCC CCCCAAGCCC AAGGACACCC TGATGATCAG CCGCACCCCC
    1021	GAGGTGACCT GCGTGGTGGT GGACGTGAGC CACGAGGACC CCGAGGTGCA GTTCAAGTGG
    1081	TACGTGGACG GCGTGGAGGT GCACAACGCC AAGACCAAGC CCCGCGAGGA GCAGTTTAAC
    1141	AGCACCTTCC GCGTGGTGTC TGTGCTGACC GTGCTGCACC AGGACTGGCT GAACGGCAAG
    1201	GAATACAAGT GCAAGGTCTC CAACAAGGCC CTGCCTGCCC CCATCGAGAA GACCATCTCT
    1261	AAGACCAAGG GCCAGCCTCG CGAGCCCCAG GTGTACACCC TGCCCCCCAG CCGCGAGGAG
    1321	ATGACCAAGA ACCAGGTGTC CCTCACCTGC CTCGTGAAGG GCTTCTACCC CAGCGACATC
    1381	GCCGTGGAGT GGGAGTCCAG CGGCCAGCCC GAGAACAACT ACAACACCAC CCCCCCCATG
    1441	CTGGACAGCG ACGGCAGCTT CTTCCTGTAC AGCAAGCTGA CCGTGGACAA GAGCCGCTGG
    1501	CAGCAGGGCA ACATCTTCTC TTGCAGCGTG ATGCACGAGG CCCTGCACAA CCGCTTCACC
	                                 Stop  NotI
    1561	CAGAAGAGCC TGAGCCTGTC CCCCGGCAAG TAATGAGCGG CCGC

SEQ ID NO: 42 (Nucleotide sequence of optimized RhD3 LC coding region)

	BamHI Kozak  Start
       1	GGATCCGCCA CCATGGACAT GCGCGTGCCC GCCCAGCTGC TGGGCCTGCT GCTGCTGTGG
      61	CTGAGGGGCG CCCGCTGCGA CATCCAGGTG ACCCAGAGCC CCAGCAGCCT GAGCGCCAGC
     121	GTGGGCGACC GCGTGACCAT CAACTGCCGC GCCAGCCAGA GCATCGGCAC CTACCTGAAC
     181	TGGTATCAGC AGAAGCCCGG CAAGGCCCCC AACCTGCTGA TCTACGCCGC CAGCTCCCTG
     241	CAGAGCGGCG TGCCCAGCCG CTTCAGCGGC AGCGGCTCCG GCACCGACTT CACCCTGACC
     301	ATCAGCAGCC TGCAGCCCGA GGACTTCGCC ACCTACTACT GCCAGCAGAC CTACAGCACC
     361	CCCACCTGGA CCTTCGGCAG GGGCACCAAG GTGGAGATCA AGCGCACCGT GGCCGCCCCC
     421	AGCGTGTTCA TCTTCCCCCC CAGCGACGAG CAGCTGAAGA GCGGCACCGC TAGCGTGGTG
     481	TGCCTGCTGA ACAACTTCTA CCCCCGCGAG GCCAAAGTGC AGTGGAAGGT GGACAACGCC
     541	CTGCAGTCCG GCAACAGCCA GGAGAGCGTC ACCGAGCAGG ACAGCAAGGA CTCCACCTAC
     601	AGCCTGAGCA GCACCCTGAC CCTGAGCAAG GCCGACTACG AGAAGCACAA GCTGTACGCC
     661	TGCGAGGTGA CCCACCAGGG CCTGTCCAGC CCCGTGACCA AGAGCTTCAA CCGCGGCGAG
	   Stop  NotI
     721	TGCTGATGAG CGGCCGC


SEQ ID NO: 43 (Optimised RhD1 HC forward primer)

5-ATCGTCTAGAGCCACCATGGACTGGACCTG-3


SEQ ID NO: 44 (Optimised RhD1 HC reverse primer)

5-ATCGGGATCCTCATCACTTGCCGGGGGAC-3

SEQ ID NO: 45 (Optimised RhD1 LC forward primer)

5-ATCGTCTAGAGCCACCATGGCCTGGGCCC-3


SEQ ID NO: 46 (Optimised RhD1 LC reverse primer)
 
5-ATCGGGATCCTCATCAGCTGCACTCGGTGGGG-3


SEQ ID NO: 47 (Sequence of the optimized RhD1 HC for insertion into pCB3 vector)

	XbaI  Kozak  Start
       1	TCTAGAGCCA CCATGGACTG GACCTGGCGC TTCCTGTTCG TGGTGGCCGC CGCCACCGGC
      61	GTGCAGAGCC AGGTGCAGCT GGTGCAGAGC GGCGCCGAGG TGAAGAAGCC CGGCAGCAGC
     121	GTCAAGGTGT CCTGCAAGGC CAGCGGCGGC ATCTTCCGCA CCTACGCCAT CTCTTGGGTC
     181	CGGCAGGCTC CCGGGCAGGG GCTCGAGTGG ATGGGCGGCA TCATCCCCAT GTTCGGCACC
     241	GTGAACTACG CCCAGAAGTT CCAGGGCCGC GTCACCATCA GCGCCGACAA GAGCACCAGC
     301	ACCGCCTACA TGGAGCTGTC CCGCCTGCGC TCCGAGGACA CCGCCGTGTA CTACTGTGCT
     361	AGGCCTCCCA GCGGCGGCTG CGGCGGCGAC TGCAGCCGCA GGGGCTACTA CTACGCTATG
     421	GACGTGTGGG GCCAGGGCAC CACCATCACC GTGAGCAGCG CTAGCACCAA GGGCCCCAGC
     481	GTGTTCCCCC TGGCCCCCAG CAGCAAGAGC ACCTCCGGCG GCACCGCCGC CCTGGGCTGC
     541	CTGGTGAAGG ACTACTTCCC CGAGCCCGTG ACCGTGAGCT GGAACAGCGG CGCCCTGACC
     601	AGCGGCGTGC ACACCTTCCC CGCCGTGCTG CAGAGCAGCG GCCTGTACAG CCTGAGCAGC
     661	GTGGTGACCG TGCCCAGCAG CAGCCTGGGC ACCCAGACCT ACATCTGCAA CGTGAACCAC
     721	AAGCCCAGCA ACACCAAGGT GGACAAGAAG GTGGAGCCCA AGAGCTGCGA CAAGACCCAC
     781	ACCTGCCCCC CCTGCCCCGC CCCCGAGCTG CTGGGCGGCC CCTCCGTGTT CCTGTTCCCC
     841	CCCAAGCCCA AGGACACCCT GATGATCAGC CGCACCCCCG AGGTGACCTG CGTGGTGGTG
     901	GACGTGAGCC ACGAGGACCC CGAGGTGAAG TTCAACTGGT ACGTGGACGG CGTGGAGGTG
     961	CACAACGCCA AGACCAAGCC CCGCGAGGAG CAGTACAACA GCACCTACCG CGTGGTGTCC
    1021	GTGCTGACCG TGCTGCACCA GGACTGGCTG AACGGCAAGG AGTACAAGTG CAAGGTCTCC
    1081	AACAAGGCCC TGCCCGCCCC CATCGAGAAG ACCATCAGCA AGGCCAAGGG GCAGCCTAGA
    1141	GAGCCCCAGG TCTACACCCT GCCTCCATCC CGCGACGAGC TGACCAAGAA CCAGGTGTCC
    1201	CTGACCTGTC TGGTCAAGGG CTTCTACCCC AGCGACATCG CCGTGGAGTG GGAGAGCAAC
    1261	GGCCAGCCCG AGAACAACTA CAAGACCACC CCCCCCGTGC TGGACAGCGA CGGCAGCTTC
    1321	TTCCTGTACA GCAAGCTGAC CGTGGACAAG AGCCGCTGGC AGCAGGGCAA CGTGTTCAGC
    1381	TGCAGCGTGA TGCACGAGGC CCTGCACAAC CACTACACCC AGAAGAGCCT GAGCCTGTCC
	         Stop   BamHI
    1441	CCCGGCAAGT GATGAGGATCC



SEQ ID NO: 48 (Sequence of the optimized RhD1 LC for insertion into pCB11 vector)
	
	XbaI  Kozak  Start
       1	TCTAGAGCCA CCATGGCCTG GGCCCTGCTG TTCCTGACCC TGCTGACCCA GGGCACCGGC
      61	AGCTGGGCCC AGAGCGCCCT GACCCAGCCC GCCAGCGTGA GCGGCAGCCC CGGCCAGTCT
     121	ATCACCATCT CTTGTAGCGG CAGCAGCAGC GACGTGGGCG GCTACAAGTA CGTGTCTTGG
     181	TATCAGCAGC ACCCCGGCAA GGCCCCCCAG CTGATGATCT ACGACGTGAA CAACCGCCCC
     241	AGCGGCGTGA GCAACCGCTT CAGCGGCTCC AAGAGCGGCA ACACCGCCAG CCTGACCATC
     301	TCTGGGCTGC AGGCTGAGGA CGAGGCCGAC TACTACTGCA GCAGCTACAC CAGCAGCTCC
     361	ACCCGCGTGT TCGGCGGCGG CACCAAGCTG ACCGTGCTGG GCCAGCCCAA GGCCGCCCCC
     421	AGCGTGACCC TGTTCCCCCC CAGCAGCGAG GAGCTCCAGG CCAACAAGGC TACCCTGGTG
     481	TGCCTGATCA GCGACTTCTA CCCCGGCGCC GTGACCGTCG CCTGGAAGGC CGACAGCAGC
     541	CCCGTGAAGG CCGGCGTGGA GACCACCACC CCCAGCAAGC AGAGCAACAA CAAGTACGCC
     601	GCCAGCAGCT ACCTGAGCCT GACCCCCGAG CAGTGGAAGA GCCACAAGAG CTACAGCTGC
	                                                              Stop
     661	CAGGTGACCC ACGAGGGCAG CACCGTGGAG AAGACCGTGG CCCCCACCGA GTGCAGCTGA
	   BamHI
     721	TGAGGATCC

	 

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